Purification:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Blocking Assay:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Recombinant:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Staining:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Enzyme-linked Immunospot:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Flow Cytometry:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Selection:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Virus:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
Expressing:Article Title: B cell-intrinsic type I interferon signaling contributes to defective antibody responses to a model antigen during persistent LCMV infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Purified anti-mouse CD16/32 (Fc Block) Clone: 2.4G2 Bio X Cell Cat# BE0307 RRID: AB_2736987 Chemicals, peptides, and recombinant proteins Perm/Wash Buffer BD BiosciencesTM Cat# 554723 BD Cytofix/Cytoperm BD BiosciencesTM Cat# 51-2090KZ BD Cytofix BD BiosciencesTM Cat# 554655 Zombie AquaTM Fixable Viability Kit BioLegend® Cat# 423102 7-AAD Viability Staining Solution BD BiosciencesTM Cat# 559925 NP(20)-PE BiosearchTM Technologies Cat#567599 NP-CGG (Chicken Gamma Globulin), Ratio >40,(Synth Scale: 5 mg) BiosearchTM Technologies Cat# N-5055E-5 NP-BSA (Chicken Gamma Globulin), Ratio 5-9,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050L NP0BSA (Chicken Gamma Globulin), Ratio >20,(Synth Scale: 50 mg) BiosearchTM Technologies Cat# N-5050H o-Phenylenediamine (OPD) Sigma-Aldrich Cat#P9029 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer Sigma-Aldrich Cat#H1009-100mL IgG1 kappa from murine myeloma SouthernBiotech Cat#M9269-1MG; Alhydrogel, 50 mL,(Size: 50 mL (5 × 10 mL)) InvivoGen Cat#VAC-ALU-50 Ethanol Greenfield Global Cat#P016EAAN Sodium azide Thermo Scientific Chemicals Cat#21610 Formaldehyde Fisher Cat#179-4 DMSO Sigma-Aldrich Cat#D8418 RPMI Wisent Cat#350-000-CL D-PBS Wisent Cat#311-425 CL Cytiva HyCloneTM Fetal Bovine Serum Fisher Cat#SH3039603 L-Glutamine Wisent Cat#609-065-EL Sodium Pyruvate Wisent Cat#600-110-EL Beta Mercaptoethanol Sigma-Aldrich Cat#M6250 Penicillin/Streptomycin Wisent Cat#450-201-EL EdU (5-ethynyl-2′-deoxyuridine) Invitrogen Cat#A10044 Critical commercial assays ELISPOT AEC Substrate Set BDTM Bioscience Cat#551951 APC BrDU Kit BD pharmingenTM Cat#552598 Click-iTTM Plus EdU Cell, Alexa FluorTM 488 Flow Cytometry Assay kit Thermo Fisher Scientific Cat#C10632 Transcription Factor Buffer Set BD pharmingenTM Cat#562574 EasySepTM Mouse Biotin Positive Selection Kit II STEMCELLTM Cat#17665 Virus strains LCMV CL13 Provided by Sam Basta N/A VSV Provided by Rolf M. Zinkernagel N/A Experimental models: Organisms/strains Ifnar1tm1Uka crossed on Cre-recombinase expression dependent on MB1 (on a C57BL/6 genetic background Provided by Ulrich Kalinke Ifnar1tm1Uka MGI: 2655303 B6(Cg)-Ifnar1tm1.1Ees/J The Jackson Laboratory Strain #:028256 RRID:IMSR_JAX:028256 B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ The Jackson Laboratory Strain #:020505 RRID:IMSR_JAX:020505 (Continued on next page) 22 Cell Reports 45, 117145, April 28, 2026 ..
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